Journal: Diabetes, Metabolic Syndrome and Obesity

Article Title: Fu-Fang-Qi-Di-Hua-Yu-Tang Improves Diabetic Macrovascular Disease via PI3K/AKT Pathway Regulation

doi: 10.2147/DMSO.S515521

Figure Lengend Snippet: Comparison of mRNA expression via the RAP1/PI3K/AKT pathway among different groups. ( a ) Relative expression of RAP 1; ( b ) Relative expression of PI3K; ( c ) Relative expression of AKT; ( d ) Relative expression of mTOR. Compared with the control (** P< 0.01), model ( # P< 0.05, ## P< 0.01), L-FFQD ( ◆ P< 0.05, ◆◆ P< 0.01), M-FFQD ( Δ P< 0.05, ΔΔ P< 0.01), and H-FFQD ( ★ P< 0.05, ★★ P< 0.01) groups, significant differences were observed.

Article Snippet: The membrane was then incubated overnight at 4°C with primary antibodies: LC3-II/I (18725-1-AP, Proteintech, USA), NF-κB p65 (10,745-1-AP, Proteintech, USA) (1:500), RAP1 (10,840-1-AP, Proteintech, USA), PI3K (20584-1-AP, Proteintech, USA), P-PI3K (bs-6417R, Bioss, China), mTOR (#2983, CST, USA) (1:1000), P-mTOR (67778-1-Ig, Proteintech, USA) (1:2000), RAGE (16346-1-AP, Proteintech, USA), P-AKT (66444-1-Ig, Proteintech, USA) (1:3000), P62 (18,420-1-AP, Proteintech, USA), AKT (60203-2-Ig, Proteintech, USA), FOX01 (18,592-1-AP, Proteintech, USA), β-actin (66009-1-Ig, Proteintech, USA), and GAPDH (10494-1-AP, Proteintech, USA) (1:5000) antibodies.

Techniques: Comparison, Expressing, Control

Journal: Diabetes, Metabolic Syndrome and Obesity

Article Title: Fu-Fang-Qi-Di-Hua-Yu-Tang Improves Diabetic Macrovascular Disease via PI3K/AKT Pathway Regulation

doi: 10.2147/DMSO.S515521

Figure Lengend Snippet: Comparison of protein expression via the RAP1/PI3K/AKT pathway among different groups. ( a ) Electrophoresis of protein expression (A. Control group; B. Model group; C. L-FFQD group; D. M-FFQD group; E. H-FFQD group; F. ATOR-MAT group); ( b ) Relative value of RAP1; ( c ) Relative value of P-PI3K/PI3K; ( d ) Relative value of P-AKT/AKT; ( e ) Relative value of P-mTOR/mTOR; ( f ) Relative value of FOX01. Compared with the control (** P< 0.01), model ( # P< 0.05, ## P< 0.01), L-FFQD ( ◆ P< 0.05, ◆◆ P< 0.01), M-FFQD ( ΔΔ P< 0.01), and H-FFQD ( ★★ P< 0.01) groups, significant differences were observed.

Article Snippet: The membrane was then incubated overnight at 4°C with primary antibodies: LC3-II/I (18725-1-AP, Proteintech, USA), NF-κB p65 (10,745-1-AP, Proteintech, USA) (1:500), RAP1 (10,840-1-AP, Proteintech, USA), PI3K (20584-1-AP, Proteintech, USA), P-PI3K (bs-6417R, Bioss, China), mTOR (#2983, CST, USA) (1:1000), P-mTOR (67778-1-Ig, Proteintech, USA) (1:2000), RAGE (16346-1-AP, Proteintech, USA), P-AKT (66444-1-Ig, Proteintech, USA) (1:3000), P62 (18,420-1-AP, Proteintech, USA), AKT (60203-2-Ig, Proteintech, USA), FOX01 (18,592-1-AP, Proteintech, USA), β-actin (66009-1-Ig, Proteintech, USA), and GAPDH (10494-1-AP, Proteintech, USA) (1:5000) antibodies.

Techniques: Comparison, Expressing, Electrophoresis, Control

Journal: Biochemical and biophysical research communications

Article Title: FAK regulates trophoblast functions of invasion and proliferation through Rap1 pathway in early-onset preeclampsia.

doi: 10.1016/j.bbrc.2025.151788

Figure Lengend Snippet: Fig. 4. FAK regulates the function of trophoblasts via the Rap1 pathway. A: KEGG analysis of Y15-treated HTR-8/SVneo cells. B: Western blotting of Rap1 in HTR8 cells treated with Y15. C: Western blotting of Rap1 in normal and EOPE placentas. A representative image is shown. D: Rap1 immunostaining of placenta from full- term normotensive pregnancies and EOPE patients. Bar = 100 μm. All statistical data were analyzed by Student’s t-test. Data are shown as mean ± SD. *P < 0.05.

Article Snippet: Primary antibodies were used to investigate these proteins: anti-FAK (1:1000, Wanleibio, China), anti-pY397FAK (1:1000, Cell Signaling Technology, USA), anti-PCNA (1:1000, Proteintech, USA), anti-Rap1(1:1000, Proteintech, USA), anti-N-cadherin (1:1000, CST, USA), and anti-β-actin (1:1000, Proteintech, USA).

Techniques: Western Blot, Immunostaining

Journal: Biochemical and biophysical research communications

Article Title: FAK regulates trophoblast functions of invasion and proliferation through Rap1 pathway in early-onset preeclampsia.

doi: 10.1016/j.bbrc.2025.151788

Figure Lengend Snippet: Fig. 5. FAK is downregulated in PE-like mouse models. A: Systolic blood pressure in L-NAME group and control group was monitored at GD 6.5, 8.5, 10.5, 12.5, 14.5, 16.5, 17.5. L-NAME: N-nitro-L-Arginine Methyl Ester. B: H&E staining of mice placentas at GD 17.5. The ratio of the placenta labyrinth zone (Lz) area to the junctional zone (Jz) area in the control group (n = 4) and L-NAME group (n = 4) was statistically calculated. Bar = 500 μm. C: HE staining of mouse kidney showed glomerular destruction in L-NAME mice (n = 3 from 3 different dams). Bar = 100 μm. D: Changes in urinary protein levels in in L- NAME (n = 5) and control (n = 5) group mouse. E: Immunofluorescence staining of FAK, pY397FAK and Rap1 in the placenta of mice at GD 17.5. Bar = 500 μm (a–c), Bar = 200 μm (d). F and G: Western blot analysis of FAK, pY397FAK and Rap1 expression in L- NAME (placenta n = 7 from 7 dams) and control (placenta n = 7 from 7 dams) group mouse placentas on GD 17.5. Statistical data were analyzed by Student’s t-test. Data are shown as mean ± SD. *P < 0.05. Dc: decidua. L-NAME: N-nitro-L-Arginine Methyl Ester.

Article Snippet: Primary antibodies were used to investigate these proteins: anti-FAK (1:1000, Wanleibio, China), anti-pY397FAK (1:1000, Cell Signaling Technology, USA), anti-PCNA (1:1000, Proteintech, USA), anti-Rap1(1:1000, Proteintech, USA), anti-N-cadherin (1:1000, CST, USA), and anti-β-actin (1:1000, Proteintech, USA).

Techniques: Control, Staining, Immunofluorescence, Western Blot, Expressing